Sean Longstaff will line up alongside his brother Matty in tonight's Checkatrade Trophy game against Notts County.
Newcastle United's Under-21s take on the League Two side at Meadow Lane (7.45pm kick-off).
The Longstaff brothers will play alongside each other in midfield for Ben Dawson's side, captained by Owen Bailey.
Coach Dawson is without goalkeeper Freddie Woodman, who is away with England's Under-21s.
Jamie Sterry is injured, while his defensive colleague Achraf Lazaar, eligible to play as an over-age player, is not involved.
In-form striker Elias Sorensen will lead the line for Newcastle, who beat League One Doncaster Rovers in their first Checkatrade Trophy game.
The final of the competition is at Wembley, and United's final group game is away to Grimsby Town on November 13.
NEWCASTLE UNITED: Harker; Cass, O'Connor, Watts, Gibson; Bailey, S Longstaff; Allan, M Longstaff, Roberts; Sorensen. Subs: Langley, Walters, Sangare, Juanito, Wilson, Longelo, Toure.