Newcastle Under-21s played in front of a competition record 7,988 fans at The Racecourse Ground which included 285 away fans.
Wrexham took the lead as Sam Dalby finally found the net after Newcastle failed to clear the danger. Dylan Stephenson had a great chance to equalise for The Young Magpies after being played in by Amadou Diallo as Wrexham led 1-0 at the break.
Both teams had chances to grab a second goal in the second half but the score remained 1-0.
In addition to Wrexham, Newcastle Under-21s are in a group alongside Port Vale and Crewe Alexandra. They face Port Vale away on October 10 and Crewe on November 14.
Here are the player ratings from the match...
13. Lucas De Bolle - n/a
On for Carlyon 76: Had a couple of touches but couldn’t help Newcastle get back into the game. | Getty Images
14. Ellis Stanton - n/a
Involved in a late altercation after leaving a foot in on Palmer but avoided punishment. | Getty Images
15. Kyle Crossley - n/a
On for Hackett 82: A late introduction as Newcastle pushed for an equaliser. | Getty Images
16. Josh Scott - n/a
On for Stephenson 82: Was alert to cut out a forward pass from Wrexham late on. | Liverpool FC via Getty Images