Newcastle Under-21s played in front of a competition record 7,988 fans at The Racecourse Ground which included 285 away fans.
Wrexham took the lead as Sam Dalby finally found the net after Newcastle failed to clear the danger. Dylan Stephenson had a great chance to equalise for The Young Magpies after being played in by Amadou Diallo as Wrexham led 1-0 at the break.
Both teams had chances to grab a second goal in the second half but the score remained 1-0.
In addition to Wrexham, Newcastle Under-21s are in a group alongside Port Vale and Crewe Alexandra. They face Port Vale away on October 10 and Crewe on November 14.
Here are the player ratings from the match...
9. Ben Parkinson - 5
Caught the eye of Eddie Howe during pre-season having played for the first-team but struggled to make an impact on this occasion. | Getty Images for Premier League
10. Amadou Diallo - 7
Posed a threat on the counter with his pace and saw a shot blocked and saved. Played a great ball over the top to Stephenson. Newcastle’s biggest threat just lacked the end product. | NW
11. Dylan Stephenson - 5
Squandered a great chance to equalise after being played in by Diallo. Saw another decent opportunity deflect wide and hit one over from a tight angle but got into some good areas with clever movement throughout. | Getty Images
12. Travis Hernes - 6
On for Huntley 67: Made his debut following his deadline day arrival. Brought some energy to the midfield. | Getty Images