Newcastle Under-21s played in front of a competition record 7,988 fans at The Racecourse Ground which included 285 away fans.
Wrexham took the lead as Sam Dalby finally found the net after Newcastle failed to clear the danger. Dylan Stephenson had a great chance to equalise for The Young Magpies after being played in by Amadou Diallo as Wrexham led 1-0 at the break.
Both teams had chances to grab a second goal in the second half but the score remained 1-0.
In addition to Wrexham, Newcastle Under-21s are in a group alongside Port Vale and Crewe Alexandra. They face Port Vale away on October 10 and Crewe on November 14.
Here are the player ratings from the match...
1. Aidan Harris - 6
Spilled the ball after initially showing good bravery in the build-up to Wrexham’s opener. Reacted well to clear the danger following a long ball over the top. Made amends for his early error by stopping Dalby in his tracks after coming off his line and did well to tip over Jones’ header in the second half. | Arsenal FC via Getty Images
2. Charlie Wiggett - 6
Did just enough to put Waters off from making it 2-0 in the second half. Photo: Laurence Griffiths
3. Ciaran Thompson - 7
Read the game well and made some important defensive blocks and contributions. | Arsenal FC via Getty Images
4. Remi Savage - 6
Played some good passes out from the back but could have done better to stop the cross in the build-up to Wrexham’s opening goal. Had to be strong in the air against physical opposition. | Getty Images for Premier League